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doi: 10.4049/jimmunol.174.12.7920 225 RapakaRRRicksDMAlcornJFChenKKhaderSAZhengMet al

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Samples were then precipitated with 1 mL absolute MeOH at –40 °C for 2 h, centrifuged (16,000 × g ), and the obtained supernatants were evaporated, and the residue was resuspended and sonicated in 100 μL 50 mM HEPES buffer (pH 7.4)